「脂質について勉強したこと（日本語）」の編集履歴（バックアップ）一覧はこちら
脂質について勉強したこと（日本語） - (2010/07/01 (木) 15:22:47) の１つ前との変更点
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*Ceramide
&bold(){}
The synthetic pathways of ceramide are two pathway.
First is the way SMase produces ceramide from SM.
Second is de nove pathway by SMS.
Acidic SMnase KO mice have a resistance for apoptosis.
Acidic SMase is localized in lysosome.
Abnormal acumulation of SM induced by delation of acidic SMase leads to Niemanpick A and B disease.
Which SM is gonna be cut by acidic SMase?
As you know, acidic SMase is localized in lysosome.
However, vesicle is released in some condition. So there is a possiblity that SMase is release to out of cell and cut the SM on the plasma membrane.
[[SMase01>http://www.j-tokkyo.com/2001/C12N/JP2001-037492.shtml]]
[[SMase02>http://wwwsoc.nii.ac.jp/jbiochem/magazine/79-12-06.pdf]]
*DAG
&bold(){}
About PC-PLC
[[PC-PLC01>http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSN-4D0YB27-11&_user=107951&_coverDate=11%2F27%2F1992&_fmt=high&_orig=browse&_sort=d&view=c&_acct=C000008318&_version=1&_urlVersion=0&_userid=107951&md5=4fe57ff08faad43b2b622376449532fc]]
[[PC-PLC02>http://www.jleukbio.org/cgi/reprint/80/2/407.pdf]]
[[PC-PLC03>http://www.pnas.org/content/95/13/7748.short]]
[[PC-PLC04>http://breast-cancer-research.com/content/12/3/R27]]
This paper is very important for me.
They use D609 and PC-PLC antibody and stain PC-PLC on the plasma membrane!
*Ceramide
&bold(){}
The synthetic pathways of ceramide are two pathway.
First is the way SMase produces ceramide from SM.
Second is de nove pathway by SMS.
Acidic SMnase KO mice have a resistance for apoptosis.
Acidic SMase is localized in lysosome.
Abnormal acumulation of SM induced by delation of acidic SMase leads to Niemanpick A and B disease.
Which SM is gonna be cut by acidic SMase?
As you know, acidic SMase is localized in lysosome.
However, vesicle is released in some condition. So there is a possiblity that SMase is release to out of cell and cut the SM on the plasma membrane.
[[SMase01>http://www.j-tokkyo.com/2001/C12N/JP2001-037492.shtml]]
[[SMase02>http://wwwsoc.nii.ac.jp/jbiochem/magazine/79-12-06.pdf]]
*DAG
&bold(){}
About PC-PLC
[[PC-PLC01>http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSN-4D0YB27-11&_user=107951&_coverDate=11%2F27%2F1992&_fmt=high&_orig=browse&_sort=d&view=c&_acct=C000008318&_version=1&_urlVersion=0&_userid=107951&md5=4fe57ff08faad43b2b622376449532fc]]
[[PC-PLC02>http://www.jleukbio.org/cgi/reprint/80/2/407.pdf]]
[[PC-PLC03>http://www.pnas.org/content/95/13/7748.short]]
[[PC-PLC04>http://breast-cancer-research.com/content/12/3/R27]]
This paper is very important for me.
They use D609 and PC-PLC antibody and stain PC-PLC on the plasma membrane!
About U73122,
U73122 is a potent inhibitor for PIP2-specific phospholipase C.
The mechanism by which U73122 inhibits this enzyme is unclear.
6microM U73122 almost completely blocks increase in Ca++ in response to ATP in fibroblast.
[[U73122-01>http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1908738/pdf/brjpharm00184-0019.pdf]]
10microM U73122 partialy blocks increase in Ca++ in response to ATP in MG6-1 cells.
[[U73122-02>http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T1W-4GWC8K1-2&_user=107951&_coverDate=11%2F15%2F2005&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1387355147&_rerunOrigin=google&_acct=C000008318&_version=1&_urlVersion=0&_userid=107951&md5=02a0690539a426479e8c71b22226bedd]]
