http://w.atwiki.jp/labmanual/ labmanual @wiki ja 2006-08-28T13:38:03+09:00 1156739883 https://w.atwiki.jp/labmanual/pages/12.html <h1>LB and LB plates</h1> <ol> <li>NaCl 10g, East extract 5g, Bactotoripton 10g, for plates, additionally, 15 g Bacto Agar<br></li> <li>Water (Deionized) 1 littler, also add 5 N NaOH 200 ul (determine the amount for each batch of East extract. to pH 7), Use magnet/stirrer to dissolve them.<br></li> <li>Autoclave<br></li> <li>Allow the solution to cool to a temperatere where you can manage to hold the bottle. (or about 60deg)<br></li> <li>Add appropriate antibiotics (e.g., ampicillin at 50or 100 ug/ml).</li> <li>Pour about 20 ml per 10cm petridish. (i.e., 1 litter gives 50 plates). You do not have to actually measure the volume. Simply pour such an amount that spreads on the entire bottom, which is about 20 ml, although depends on the temperature (i.e., viscosity of the solution).<br></li> <li>Let them cool to r.t. and store in the cold room (4 deg).</li> </ol> <br> <br> 2006-08-28T13:38:03+09:00 1156739883 https://w.atwiki.jp/labmanual/pages/2.html 2006-08-28T13:28:44+09:00 1156739324 https://w.atwiki.jp/labmanual/pages/11.html <br> <br> <br> <h1>Useful links</h1> <ol> <li>Sutter <a href= "http://www.sutter.com/contact/faqs/pipette_cookbook.pdf">Pipette Cookbook</a>:tells you how to make pipettes with desired shapes.<br></li> </ol> <br> 2006-08-25T05:48:17+09:00 1156452497 https://w.atwiki.jp/labmanual/pages/9.html <br> <h1>Oligonucleotides</h1> <ol> <li>MWG (<a href="https://ecom.mwgdna.com/register/index.tcl">oligo orderpage</a>): requires registration and P.O. number.<br></li> </ol> <br> <h1>Equipments<br></h1> <ol> <li><a href="http://online.projectordoctor.com/">Projector Doctor</a>:Takescareof old projectors in the U.S.<br></li> </ol> 2006-08-25T05:28:52+09:00 1156451332 https://w.atwiki.jp/labmanual/pages/4.html 2006-08-25T05:18:02+09:00 1156450682 https://w.atwiki.jp/labmanual/pages/10.html <br> <br> <h1><br></h1> <ol> <li>九大医　<a href= "http://www.bioreg.kyushu-u.ac.jp/saibou/index.html">中山敬一</a>：　<a href="http://www.bioreg.kyushu-u.ac.jp/saibou/message/message.html">教授からのメッセージ</a>（心構え、心がけ、その他）</li> </ol> <br> 2006-08-24T02:42:01+09:00 1156354921 https://w.atwiki.jp/labmanual/pages/8.html <h1>Companies</h1> <ol> <li>http://www.cocalicobiologicals.com/</li> </ol> <br> <br> <h1>Examples of Immunization Schedule<br></h1> Note: After giving a boost, the antibody titer will increase and then decease. Typically, the animals should be sacrificed between 10-14 days after the final boost. If one month has past, another boost before killing the animal is recommended.<br> <br> 2006-08-22T04:44:40+09:00 1156189480 https://w.atwiki.jp/labmanual/pages/6.html 2006-08-22T03:49:51+09:00 1156186191 https://w.atwiki.jp/labmanual/pages/7.html 2006-08-17T11:52:19+09:00 1155783139 https://w.atwiki.jp/labmanual/pages/1.html 2006-08-17T10:56:22+09:00 1155779782