from Bio.Seq import Seq
from Bio.SeqUtils.MeltingTemp import Tm_staluc
from Bio.Alphabet import generic_nucleotide
from Bio.SeqUtils import GC
#INPUT
mer = 'GCCGGGTACCATGGTCTTGCTTACAACATT'
#OUTPUT
num = 20
while num <= 30:
i = mer[0:num]
s = Seq(i, generic_nucleotide)
print ("%s mer" % num),
print i
print("TM: %0.2f℃" % Tm_staluc(s))
print("GC: %0.2f" % GC(s))
num = num + 1
