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Maize Phosphoenolpyruvate Carboxylase
Phosphoenolpyruvate carboxylases (PEPC, EC 4.1.1.31) from higher plants are regulated by both allosteric effects and reversible phosphorylation. Previous x-ray crystallographic analysis of Zea mays PEPC has revealed a binding site for sulfate ion, speculated to be the site for ...
2009
Bacterial-type phosphoenolpyruvate carboxylase (PEPC) functions as a catalytic and regulatory subunit of the novel class-2 PEPC complex of vascular plants
Phosphoenolpyruvate carboxylase (PEPC) is a tightly regulated anaplerotic enzyme situated at a major branch point of the plant C metabolism. Two distinct oligomeric classes of PEPC occur in the triglyceride-rich endosperm of developing castor oil seeds (COS). Class-1 PEPC is ...
2008
Oxidative pentose phosphate pathway-dependent sugar sensing as a mechanism for regulation of root ion transporters by photosynthesis
Root ion transport systems are regulated by light and/or sugars, but the signaling mechanisms are unknown. We showed previously that induction of the NRT2.1 NO 3 – transporter gene by sugars was dependent on carbon metabolism downstream hexokinase (HXK) in ...
Evolution of C4 phosphoenolpyruvate carboxylase in Flaveria: determinants for high tolerance towards the inhibitor L-malate
During the evolution of angiosperms, C 4 phosphoenolpyruvate carboxylases have evolved several times independently from ancestral non-photosynthetic isoforms. They show distinct kinetic and regulatory properties when compared with the C 3 isozymes. To identify the ...
Kinetic analyses of recombinant isoforms of phosphoenolpyruvate carboxylase from Hydrilla verticillata leaves and the impact of substituting a C4-signature serine
Hydrilla verticillata (Lf) Royle is a single-cell C 4 NADP-malic enzyme species in which the C4 and Calvin cycles operate in the same cell; phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) is the initial carboxylase. The cDNAs of two Hydrilla PEPC isoforms were ...
Maize C4-form phosphoenolpyruvate carboxylase engineered to be functional in C3 plants: mutations for diminished sensitivity to feedback inhibitors and for …
Introducing a C 4 -like pathway into C 3 plants is one of the proposed strategies for the enhancement of photosynthetic productivity. For this purpose it is necessary to provide each component enzyme that exerts strong activity in the targeted C 3 plants. Here, a maize C 4 -form ...
2007
Physiological impacts of modulating phosphoenolpyruvate carboxylase levels in leaves and seeds of Arabidopsis thaliana
Arabidopsis thaliana was transformed with a Sorghum C 4 PEPC cDNA under the control of the 35S promoter. Primary transformants were isolated and classified on a seed PEPC activity basis: A lines (two-fold increase) and B lines (four-fold increase). Primary transformants ...
2006
Light regulation of the photosynthetic phosphoenolpyruvate carboxylase (PEPC) in Hydrilla verticillata
The submersed monocot, Hydrilla verticillata (Lf) Royle, is a facultative C 4 NADP-malic enzyme (NADP-ME) plant in which the C 4 and Calvin cycles co-exist in the same cell. Futile cycling is avoided by an intracellular separation of carboxylases between the cytosol and ...
The importance of the strictly conserved, C-terminal glycine residue in phosphoenolpyruvate carboxylase for overall catalysis: Mutagenesis and truncation of Gly-961 …
Phosphoenolpyruvate carboxylase (PEPC) is a “multifaceted”, allosteric enzyme involved in C4-acid metabolism in green-plants/- microalgae and prokaryotes. Prior to the elucidation of the three-dimensional structures of maize C4-leaf and Escherichia coli PEPC, our ...
2005
In vitro proteolysis of phosphoenolpyruvate carboxylase from developing castor oil seeds by an endogenous thiol endopeptidase
Two novel phosphoenolpyruvate carboxylase (PEPC) isoforms have been biochemically characterized from endosperm of developing castor oil seeds (COS). The association of a 107 kDa PEPC subunit (p107) with an immunologically unrelated bacterial PEPC-type 64 kDa polypeptide leads ...
最終更新：2010年05月28日 12:11
