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Metabolic network analysis
Metabolic network analysis is a tool for investigating the features that identify the topology of a metabolic network and the relative activities of its individual branches. The pillars of metabolic network analysis are mathematical modeling, allowing for a quantitative analysis, ...
Metabolic network analysis of the causes and evolution of enzyme dispensability in yeast
Under laboratory conditions 80% of yeast genes seem not to be essential for viability 1 . This raises the question of what the mechanistic basis for dispensability is, and whether it is the result of selection for buffering or an incidental side product. Here we analyse these issues ...
Genealogy profiling through strain improvement by using metabolic network analysis: metabolic flux genealogy of several generations of lysine-producing …
A comprehensive approach of metabolite balancing, 13 C tracer studies, gas chromatography-mass spectrometry, matrix-assisted laser desorption ionization-time of flight mass spectrometry, and isotopomer modeling was applied for comparative metabolic ...
Metabolic network analysis of Penicillium chrysogenum using 13C-labeled glucose
To provide a rational approach for directed genetic alter- ations in microorganisms, often referred to as metabolic engineering (Bailey, 1991; Stephanopoulos, 1999; Yarmush and Berthiaume, 1997), a detailed map of the fluxes in the central metabolism is an essential ...
Metabolic network analysis of Bacillus clausii on minimal and semirich medium using 13C-labeled glucose
Using 13 C-labeled glucose fed to the facultative alkalophilic Bacillus clausii producing the alkaline serine protease Savinase, the intracellular fluxes were quantified in continuous cultivation and in batch cultivation on a minimal medium. The flux through the pentose phosphate ...
Genome-scale metabolic network analysis of the opportunistic pathogen Pseudomonas aeruginosa PAO1
Pseudomonas aeruginosa is a major life-threatening opportunistic pathogen that commonly infects immunocompromised patients. This bacterium owes its success as a pathogen largely to its metabolic versatility and flexibility. A thorough understanding of P. aeruginosa's ...
A metabolic network stoichiometry analysis of microbial growth and product formation
... Other work on metabolic network analysis using linear op- timization techniques has been performed on hybridoma cell Esehenchiu coli metabolism,3' .32 and acetate overflow in E. c01i.""~ Following the approach to solve underdetermined metabolic networks by conducting ...
Metabolic network analysis of lysine producing Corynebacterium glutamicum at a miniaturized scale
Abstract: We present a straightforward approach com- prising 13C tracer experiments at 200-AL volume in 96-well microtiter plates with on-line measurement of dissolved oxygen for quantitative high-throughput metabolic net- work analysis at a miniaturized scale. This ...
Large-scale 13C-flux analysis reveals mechanistic principles of metabolic network robustness to null mutations in yeast
最終更新：2010年09月28日 21:11
