| Team | Constructor | Motorcycle | No | Rider | Rounds | |
|---|---|---|---|---|---|---|
| Repsol Honda Team | Honda | Honda RC212V | 4 | ITA | Andrea Dovizioso | 1 |
| 26 | ESP | Dani Pedrosa | 1 | |||
| Monster Yamaha Tech 3 Team | Yamaha | Yamaha YZR-M1 | 5 | USA | Colin Edwards | 1 |
| 11 | USA | Ben Spies | 1 | |||
| Interwetten Honda MotoGP Team | Honda | Honda RC212V | 7 | JPN | Hiroshi Aoyama | 1 |
| LCR Honda MotoGP | Honda | Honda RC212V | 14 | FRA | Randy de Puniet | 1 |
| Rizla Suzuki MotoGP | Suzuki | Suzuki GSV-R | 19 | ESP | Álvaro Bautista | 1 |
| 65 | ITA | Loris Capirossi | 1 | |||
| Ducati Marlboro Team | Ducati | Ducati Desmosedici GP10 | 27 | AUS | Casey Stoner | 1 |
| 69 | USA | Nicky Hayden | 1 | |||
| San Carlo Honda Gresini Team | Honda | Honda RC212V | 33 | ITA | Marco Melandri | 1 |
| 58 | ITA | Marco Simoncelli | 1 | |||
| Pramac Racing Team | Ducati | Ducati Desmosedici GP10 | 36 | FIN | Mika Kallio | 1 |
| 41 | ESP | Aleix Espargaró | 1 | |||
| Páginas Amarillas Aspar Racing Team | Ducati | Ducati Desmosedici GP10 | 40 | ESP | Héctor Barberá | 1 |
| Fiat Yamaha Team | Yamaha | Yamaha YZR-M1 | 46 | ITA | Valentino Rossi | 1 |
| 99 | ESP | Jorge Lorenzo | 1 |
