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In vivo analysis of intracellular amino acid labelings by GC/MS.
C Wittmann, M Hans, E Heinzle - Analytical biochemistry, 2002 - mendeley.com
... TBDMS derivatization of amino acids is especially useful in metabolic network analysis, because M-57 fragments containing the entire carbon skeleton of the analyte can be observed by GC/MS with high signal intensities 8. The potential of the method is exemplified for ...
Comparative metabolic flux analysis of lysine-producing Corynebacterium glutamicum cultured on glucose or fructose
nih.gov [HTML]P Kiefer, E Heinzle, O Zelder, C … - Applied and …, 2004 - Am Soc Microbiol
... A comprehensive approach to 13 C tracer studies, labeling measurements by gas chromatography-mass spectrometry, metabolite balancing, and isotopomer modeling, was applied for comparative metabolic network analysis of lysine-producing Corynebacterium glutamicum ...
It is all about metabolic fluxes
... I. Experimental observations. Biotechnol. Bioeng. 55:305-316.[CrossRef] 21; Thykaer, J., B. Christensen, and J. Nielsen. 2002.
Metabolic network analysis of an adipoyl-7-ADCA producing strain of Penicillium chrysogenum: elucidation of adipate degradation.Metab. Eng. ...
Using topology of the metabolic network to predict viability of mutant strains
... Ph.D. Thesis. University of Leeds; 1994. 14. Papp B, Pal C, Hurst LD: Metabolic network analysis of the causes and evolution of enzyme dispensability in yeast. Nature 2004, 429(6992):661-664. 15. Jeong H, Tombor B, Albert R, Oltvai ZN, Barabasi AL: The large-scale ...
Novel proteins, putative membrane transporters, and an integrated metabolic network are revealed by quantitative proteomic analysis of Arabidopsis cell …
... This is aided by a metabolic network analysis that reveals a tight integration of functions and highlights specific metabolite nodes that most probably represent entry and exit metabolites that could require transport across the peroxisomal membrane. ...
High-throughput phenomics: experimental methods for mapping fluxomes
... From its early days when material fluxes were balanced within assumed reaction networks [7.], metabolic network analysis [6. and 8.] has matured to actually identify the topology of active reactions and pathways and to quantify the molecular flux through them on a variety of ...
Use of genome-scale microbial models for metabolic engineering
... incorporate genome-scale biological data. Genome-scale stoichiometric models of microorganisms represent a first step in this direction. FBA, flux balance analysis; MFA, metabolic flux analysis; MNA, metabolic network analysis. ...
Network identification and flux quantification in the central metabolism of Saccharomyces cerevisiae under different conditions of glucose repression
... flux estimates. The use of 13 C-labeled substrates enables both identification of the metabolic network structure and quantification of the metabolic fluxes and is therefore referred to as metabolic network analysis (5). This kind ...
Metabolic flux analysis of Escherichia coli K12 grown on 13C-labeled acetate and glucose using GC-MS and powerful flux calculation method
Structural and functional analysis of cellular networks with CellNetAnalyzer
... CNA extends its predecessor FluxAnalyzer, originally developed for metabolic network analysis[7], by new methods for signalling and regulatory networks, ie for networks where signal flows are dominating (in contrast to mass flows in metabolic networks). ...
最終更新：2010年09月28日 22:16
