Microtube Assay
1. For 10 samples, add 25 ul of DC reagent S to 1250 ul of DC reagent A to make reagent A'
2. Pipet 0~25 ul of BSA standard and samples to microtubes (replication recommended)
3. Add 125 ul RC reagent I, vortex, incubate for 1 min at rt
4. Add 125 ul RC reagent II, votex
5. Centrifuge at 15,000xg for 5 min
6. Discard Sup. To Ppt, add 127 ul reagent A', vortex for 5 min
7. Add 1 ml of DC reagent B, vortex immediately, incubate for 15 min at rt
8. Read A750 (stable at least 1 h)
